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EGCG inhibits the intrinsic ATPase activity of Hsp70. The effect of EGCG on the basal ATPase activities of (A) PfHsp70-1 and (B) PfHsp70-z were investigated. The released Pi was monitored at 595 nm using a direct colourimetric assay. The experiment was carried out under ATP saturation (5 mM) in the presence of 0.4 µM PfHsp70-1/PfHsp70-z.
