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. 2017 Dec 5;22(12):2139. doi: 10.3390/molecules22122139

Figure 5.

Figure 5

EGCG abrogates interaction of PfHsp70-1 with both PfHsp70-z and PfHop. Shown are the representative SPR sensorgrams representing the interaction of PfHsp70-1/PfHsp70-1NBD and PfHsp70-z as investigated under varying levels of EGCG. The data represent global fitting obtained under conditions in which ligand and analyte were interchanged (A). The assay was repeated for PfHsp70-1/PfHsp70-1NBD (as ligand) versus PfHsp70-z (as analyte) (B); and PfHsp70-1 (ligand) versus PfHop (analyte) (C). The assay was conducted under the following conditions: absence of nucleotides (NN); presence of either 5 mM ATP/ADP or 2.5 μM EGCG, respectively. The relative affinities for interaction of the following protein pairs are shown: PfHsp70-1-PfHsp70-z (A1); PfHsp70-1NBD-PfHsp70-z (B1); and PfHsp70-1-PfHop (C1). The relative affinities shown represent logKD normalized to logKDNN in each case.