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. 2017 Dec 5;22(12):2139. doi: 10.3390/molecules22122139

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© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Normalized dose response curve for the in vitro susceptibility of P. falciparum to EGCG. The in vitro antiplasmodial activity of EGCG was investigated using a pLDH ELISA assay. The assay was conducted on P. falciparum 3D7 cells maintained at the blood stages in the presence of EGCG. Chloroquine was used as a control (IC₅₀ of 8.5 nM). The curves represent data obtained from assays conducted in the presence of choloroquine (A) or EGCG (B), respectively. IC₅₀ values were calculated from the generated dose response curves obtained using GraphPad Prism 6. The experiment was performed in duplicate wells. Standard deviations obtained from three replicate assays are shown.