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. 2017 Dec 18;22(12):2263. doi: 10.3390/molecules22122263

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© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Acanthamoeba castellanii NCKH_D secreted proteins induced cell damage by disruption of C6 cells. The observation of C6 cell co-incubated with Acanthamoeba castellanii NCKH_D secreted proteins for 2 h by CPE assay (a) and time-lapse microscopy (c); (b) Quantification of the coverage of C6 cells after co-incubated with secreted proteins for 2 h; (c) The morphology of C6 cells in control and Asp treated groups by time-lapse microscopy. Those results were means ± S.D. (standard deviation) of triplicate independent experiments. *** p < 0.01. Scale bar = 50 μm.

Acanthamoeba castellanii NCKH_D secreted proteins induced cell damage by disruption of C6 cells. The observation of C6 cell co-incubated with Acanthamoeba castellanii NCKH_D secreted proteins for 2 h by CPE assay (a) and time-lapse microscopy (c); (b) Quantification of the coverage of C6 cells after co-incubated with secreted proteins for 2 h; (c) The morphology of C6 cells in control and Asp treated groups by time-lapse microscopy. Those results were means ± S.D. (standard deviation) of triplicate independent experiments. *** p < 0.01. Scale bar = 50 μm.