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. 2017 Nov 27;22(12):2068. doi: 10.3390/molecules22122068

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© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Increase of mineralization in pentamidine-treated zebrafish. (A) The gross morphology of zebrafish aged at 7 dpf which have been treated with different concentrations of pentamidine (10, 20, and 30 µM, left bright-filed panel) from 3 dpf onwards. Calcein staining on control and pentamidine-treated embryos at 7 dpf (right green fluorescent panel); (B) Quantification of mineralization degree detecting the fluorescence intensity at the area of centrum form ring in the notochord. (values are mean ± SD; tested by one-way ANOVA pairwise comparison; N = fish number; Different letters indicate significant differences) (C) Chemical structure of pentamidine.