Table 2.
Identification and quantification data for the 8 phenolic compounds isolated from black spruce bark extract.
| Identification | Quantification | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Comp. | Ret. Time (min) | λmax (nm) | Exact Mass | Formula | Suggested Compound | Regression Equation | R2 | Linear Range ug/mL | LOD | LOQ | Concentration Range mg/100 g Dry Extracts |
| 1 | 12.4 | 290 | 326.0994 | C15H18O8 | trans-p-coumaric acid β-d-glucopyranoside | y = 2140.2x + 8.2609 | 0.9997 | 5–500 | 0.1 | 0.4 | 160–265 |
| 2 | 13.9 | 290, 315 | 356.1093 | C16H20O9 | trans-ferulic acid β-d-glucopyranoside | y = 1486.4x − 60.765 | 0.9987 | 5–1000 | 0.4 | 1.3 | 894–1073 |
| 3 | 20.2 | 325, 305 | 406.1243 | C20H21O9 | trans-astringin | y = 2284x − 441.91 | 0.999 | 5–1000 | 3.8 | 11.4 | 2272–4613 |
| 4 | 26.8 | 305, 320 | 390.1303 | C20H22O8 | trans-piceid | y = 3384.1x − 528.13 | 0.9971 | 5–500 | 0.2 | 0.6 | 1805–3094 |
| 5 | 29.4 | 325, 303, 290 | 420.1416 | C21H24O9 | trans-isorhapontin | y = 2944.6x − 265.82 | 0.9979 | 1–1000 | 0.6 | 1.9 | 4256–11971 |
| 6 | 37.3 | 330, 310, 283 | 838.2684 | C42H46O18 | piceaside O and P | y = 1056.4x − 329.29 | 0.9933 | 1–1000 | 0.4 | 1.2 | 3508–4853 |
| 7 | 38.5 | 305, 320 | 228.0796 | C14H12O3 | trans-resveratrol | y = 7520.7x + 43.49 | 0.9914 | 1–500 | 0.2 | 0.6 | 53–302 |
| 8 | 40.3 | 325, 303, 290 | 258.0892 | C15H14O4 | trans-isorhapontigenin | y = 2062.4x − 415.08 | 0.9979 | 5–1000 | 0.3 | 0.9 | 2257–3654 |
Comp.: compound number; Ret. time: retention time; λmax: maximal wavelength value; Exact mass calculated from m/z (mass-to-charge ratio); R2: coefficient of determination; LOD: Limit of detection, LOQ: Limit of quantification.