Figure 7.

The effects of steppogenin (1) on nitrite (A) production and iNOS and COX-2 expression (B) in lipopolysaccharide (LPS)-stimulated primary rat microglial cells. (A,B) The cells were pretreated for 3 h with the indicated concentrations of 1 and then stimulated for 24 h with LPS (1 μg/mL). The data are presented as the mean ± SD of three experiments. The band intensities were quantified by densitometry and normalized to the intensities of the β-actin band; the normalized values are presented below each band. ** p < 0.01; *** p < 0.001 compared with the LPS-treated group.