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. 2018 Sep 21;193(3):327–340. doi: 10.1111/cei.13153

Figure 1.

Figure 1

Human epididymis protein 4 (HE4) down‐regulates the expression of osteopontin (OPN) in peripheral blood mononuclear cells (PBMCs). (a) Two‐colour flow cytometric analysis of PBMC following a 24‐h incubation with 0·01 μg/ml of recombinant human epididymis protein 4 (rHE4) or vehicle [control (CTR)]. Two‐dimensional scatterplots of OPN (AlexaFluor 647) and CD3 [fluorescein isothiocyanate (FITC)] are shown. The numbers on the scatterplots represent mean ± standard error of the mean (s.e.m.) % of each quadrant. (b) OPN transcription in response to a 6‐h incubation with 0.01 μg/mL rHE4 (HE4) or vehicle (CTR) were evaluated by real time polymerase chain reaction (PCR). A bar graph represents relative expression levels against control. (c) OPN concentrations of PBMC lysates and culture supernatants after a 24‐h incubation with 0·01 μg/ml of rHE4 or vehicle (CTR) were evaluated by enzyme‐linked immunosorbent assay (ELISA). All the experiments were performed with PBMCs from four individual donors and repeated three (a), nine (b) and 10 (c) times. The mean is shown in the bar graphs; error bars represent s.e.m. (n > 10). *P < 0·05; **P < 0·01.