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Values of ΔTm for ligand interaction with F21T/K+ (A), F21T/Na+ (B) and FmycT/K+ (C), determined via FRET melting assays. Ligands 14 (yellow), 13 (red) and 10 (cyan) were tested at 1 µM final concentration, with the G4-DNA fixed at 0.2 µM and the competing ds26 duplex DNA at 0, 2 or 5 µM. Buffer used was 10 mM lithium cacodylate (pH 7.2), supplemented with: 90 mM LiCl/10 mM KCl in (A); 90 mM LiCl/10 mM NaCl in (B); and 99 mM LiCl/1 mM KCl in (C).
