Table 1.

Linear relation between peak area and concentration.

Analytes	Regression Equation	r2	Linear Range (μg/mL)	LOD (μg/mL)	LOQ (μg/mL)
Chlorogenic acid	y = 20.427x − 20.31	0.9995	2.89–289.4	0.053	0.178
Mangiferin	y = 81.058x − 38.27	0.9996	1.16–116.4	0.014	0.047
Isomangiferin	y = 61.572x − 46.64	0.9997	2.29–228.8	0.030	0.101
Trifolin	y = 32.979x − 16.29	0.9996	0.96–95.9	0.085	0.284
Astragalin	y = 42.194x − 18.94	0.9996	0.99–98.6	0.047	0.155

In the regression equation y = ax + b, x refers to the concentration of analytes (μg/mL); y is the peak area; and r² is the correlation coefficient of the equation; LOD is the limit of detection; LOQ is the limit of quantification.