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(a) Structures of 5′-bispyrene-modified nucleotide monomers 5′Nbpyr and the quencher of fluorescence, BHQ1; (b) The fluorescence emission spectra of MB containing 5′Nbpyr monomer (B: guanin-9-yl) [85]. A: adenin-9-yl, U: uracil-1-yl, C: cytidin-1-yl, and G: guanin-9-yl.
