FIGURE 4.

Knocking down LRRC19 suppresses NFκB signaling. Western blot analysis of NFκB signaling components. Primary dermal fibroblasts transfected with either scramble siRNA or LRRC19 siRNA were challenged with 50 ng/ml IL-1β for the indicated time points. Cell lysate after treatment was subjected to SDS-PAGE to determine the level of A) total p65, phospho-p65 (S536), total IkBα and phosphor-IkBα (S32); or the level of B) COX-2 and iNOS. The band intensity of each target protein was normalized against its corresponding loading control. The normalized band densitometry of the siRNA control transfected sample at day 0 was set as baseline of value 1. The data are representative of at least three independent experiments.