Table 1.
Description of PCR and RFLP products of P53 and CD95 genes.
Gene | SNPs | PCR amplicon | RFLP (R.E.) | Genotypes | Product sizes (bp) |
---|---|---|---|---|---|
P53 | +72 G/C (Arg72Pro) | 199 bp | BstUI (Bsh1236I) | +72 G/G | 113 + 86 |
+72 C/C | 199 | ||||
+72 G/C | 199 + 113 + 86 | ||||
CD95 | −670 A/G | 233 bp | MvaI (BstNI) | −670 A/A | 233 + 99 |
−670 G/G | 189 + 99 + 44 | ||||
−670 A/G | 233 + 189 + 99 + 44 |
SNPs: single nucleotide polymorphisms.
RFLP: restriction fragment length polymorphism.
R.E.: restriction enzyme.