Table 3.
Validation of method for identifying six PhGs by UPLC-PDA.
| Analyte | Precision (RSD, %) | Repeatability | Stability | Recovery (%, n = 3) | ||
|---|---|---|---|---|---|---|
| Intraday (n = 6) | Interday (n = 6) | (RSD, %, n = 6) | (RSD, %, n = 6) | Mean | RSD (%) | |
| Echinacoside | 0.55 | 1.44 | 1.37 | 1.90 | 98.80 | 0.86 |
| Cistanoside A | 0.99 | 1.40 | 0.78 | 0.94 | 100.10 | 2.23 |
| Acteoside | 0.95 | 1.52 | 1.92 | 1.71 | 102.20 | 1.33 |
| Isoacteoside | 1.32 | 1.50 | 1.11 | 0.92 | 97.60 | 2.13 |
| 2′-Acetylacteoside | 0.68 | 1.26 | 1.88 | 1.59 | 101.20 | 1.10 |
| Tubuloside B | 0.79 | 1.77 | 1.06 | 1.89 | 99.10 | 2.92 |
RSD = (SD/mean) × 100%.; Recovery = (amount found − original amount)/spiked amount × 100%.