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. 2017 Nov 17;22(11):1997. doi: 10.3390/molecules22111997

Figure 4.

Figure 4

RAGE/HMGB1 binding in LMWH-treated vs. untreated placental villous explants. HMGB1 protein levels were evaluated by western blot after immunoprecipitation with RAGE antibody of protein lysates from placental villous explants treated by 0.5 U of LMWH for 24 h and 48 h. (A) Representative WB for HMGB1 protein levels after RAGE IP at 24 h (ctrl n = 16 and 0.5 U LMWH n = 15) and 48 h (ctrl n = 5 and 0.5 U LMWH n = 6); (B) Representative WB for HMGB1 protein levels in RAGE IP supernatant at 24 h (ctrl n = 8 and 0.5 U LMWH n = 10) and 48 h (ctrl n = 5 and 0.5 U LMWH n = 6). Statistical significance (*) has been considered as p < 0.05.