Figure 3.

Typical results of detecting GNAS mutation in three patients with suspect McCune–Albright syndrome or fibrous dysplasia. The PNA probe assay generated melting curves of PCR products of the samples (top). The PCR products (marked as clamping PCR) were analyzed by Sanger sequencing (middle). For comparison, sequencing results from PCR products without using PNA probe (marked as conventional PCR) are also shown (bottom). Open arrowheads indicate the position of mutant bases or mutant peaks; filled arrowheads indicate the wild-type peaks.