Table 1.
Protein carbonyls, histidine residues, lysine residues, available lysine, sulfhydryl and disulfide groups contents of β–conglycinin exposed to hydroxyl radical–generating systems with increasing concentrations of H2O2 for 3 h at room temperature.
| H2O2 (mmol/L) | Carbonyls (nmol/mg) | Histidine (g/100 g) | Lysine (g/100 g) | Available Lysine (g/100 g) | Free SH (nmol/mg) | Disulfide Groups (nmol/mg) |
|---|---|---|---|---|---|---|
| 0 | 8.02 ± 0.29 e | 1.17 ± 0.01 a | 2.91 ± 0.02 a | 6.23 ± 0.08 a | 8.66 ± 0.04 a | 84.89 ± 2.28 bc |
| 0.1 | 8.14 ± 0.17 e | 1.16 ± 0.02 a | 2.89 ± 0.03 a | 5.96 ± 0.08 b | 8.58 ± 0.01 b | 81.42 ± 3.17 cd |
| 0.5 | 8.36 ± 0.27 e | 1.15 ± 0.01 ab | 2.82 ± 0.02 b | 5.44 ± 0.14 c | 7.42 ± 0.03 c | 79.25 ± 1.77 d |
| 1 | 9.18 ± 0.26 d | 1.13 ± 0.01 b | 2.79 ± 0.03 b | 5.06 ± 0.11 d | 5.18 ± 0.02 d | 88.44 ± 3.36 b |
| 5 | 10.95 ± 0.20 c | 1.10 ± 0.02 c | 2.71 ± 0.03 c | 4.29 ± 0.17 e | 1.36 ± 0.05 e | 89.13 ± 3.39 b |
| 10 | 13.73 ± 0.25 b | 1.08 ± 0.03 c | 2.62 ± 0.04 d | 4.09 ± 0.06 f | 1.01 ± 0.07 f | 95.18 ± 1.31 a |
| 15 | 14.45 ± 0.18 a | 1.04 ± 0.01 d | 2.54 ± 0.03 e | 3.68 ± 0.13 g | 0.95 ± 0.04 g | 96.24 ± 3.21 a |
All data points are represented as the mean of triplicate determinations ± standard errors. Values in the same column followed by different superscript letters (a–g) are significantly different (p < 0.05), which was concluded by SPSS software. In statistical analysis process, the effect of random error can be compared by probability value (p). If p < 0.05, it means that the effect of random error could be ignored and the difference of the value in the Table 1 are significative. Otherwise, the values in the same column followed by or within the same superscript letters are not significantly different (p > 0.05).