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. 2017 Nov 5;22(11):1904. doi: 10.3390/molecules22111904

Figure 2.

Figure 2

Characteristics of iron content in the inner cavity of Dps. (A) and (B) TEM images of inorganic particles taken from the native protein (1.64 μM with 126 Fe-ions per dodecamer) and Dps, loaded with 1 mM of Fe(NH4)2(SO4)2, respectively. Particles accounted for image analysis are countered by Altami Studio software (http://altamisoft.ru/products/altami_studio/). An insert in panel A shows an unprocessed image identical to the bottom part of the same panel. (C) apo-form of the Dps, prepared as described in Materials and Methods. (D) Image of 1 mM of Fe(NH4)2(SO4)2 alone. Scaling is identical for all images and is shown in (B). (E) Densitograms showing the size distribution of inorganic particles calculated with a resolution step of 0.2 nm. Dashed and solid lines correspond to native and iron-loaded proteins, respectively. N denotes the number of analyzed particles, d—average diameter of cores approximated by circles, σ—StD of d. (F–H) Mössbauer spectra obtained for αFe, 1 mg of dry Fe(NH4)2(SO4)2 and for the Dps protein incubated with 1 mM Fe(NH4)2(SO4)2.