Table 4.

Stability of the seven analytes in rat plasma (n = 3).

Compounds	Concentration (ng/mL)	Room Temperature for 4 h		Autosampler for 24 h		−20 °C for 3 Weeks		Freeze-Thaw Cycles
		Remains (%)	RSD (%)	Remains (%)	RSD (%)	Remains (%)	RSD (%)	Remains (%)	RSD (%)
aurantio-obtusin	1.88	103.7	4.62	104.8	5.54	104.49	5.13	106.89	8.40
	18.8	97.1	5.86	105.8	6.86	102.69	7.42	105.24	6.69
	94	95.4	7.65	97.8	4.58	97.84	5.17	98.05	4.43
obtusifolin	2.05	98.3	5.27	102.4	6.46	101.59	6.93	103.98	5.11
	20.5	91.5	5.93	93.4	7.39	93.69	6.73	95.74	5.81
	103	98.2	4.53	98.3	5.42	99.49	5.03	101.69	4.31
questin	1.68	99.0	4.03	100.8	4.72	98.14	5.42	103.45	3.78
	16.8	99.6	2.80	102.8	6.99	102.44	4.92	104.83	6.45
	84	105.5	6.37	99.1	4.40	103.54	2.41	96.70	5.09
SC-1	2.07	95.0	6.87	97.1	8.70	98.29	7.87	100.56	4.81
	20.7	97.9	4.06	99.4	4.76	99.89	4.46	102.14	7.81
	103	105.9	5.32	99.3	6.53	103.84	5.99	97.01	8.16
rhein	2.13	106.2	9.77	103.0	8.99	102.84	9.07	105.23	7.15
	21.3	94.9	4.47	95.9	5.30	96.64	4.93	98.77	4.29
	107	102.6	4.95	102.7	6.01	103.89	5.54	106.25	7.76
emodin	2.07	95.8	5.27	106.8	6.46	102.54	6.93	101.15	4.11
	20.7	99.3	4.58	102.2	5.48	101.99	5.08	96.36	4.36
	103	104.5	7.59	104.0	6.69	103.49	6.67	105.71	6.23
SC-2	1.80	105.5	4.74	93.5	5.71	100.74	5.28	95.65	4.53
	18	94.9	6.34	101.2	5.14	99.29	4.79	101.66	4.10
	90	105.5	5.96	103.6	7.43	106.29	6.77	97.54	5.81