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. 2017 Nov 22;22(11):2030. doi: 10.3390/molecules22112030

Figure 2.

Figure 2

The effects of the three extracts and three compounds isolated from V. trifolia on triglycerol levels in 3T3-L1 cells. The 3T3-L1 cells were cultured in 24-well plates and differentiated under the conditions described in the materials and methods section for each compound. Undifferentiated cells, cells with the addition of the MDI mixture (a mixture of 0.5 mM 3-isobutyl-1-methylxanthine (M), 0.1 μM dexamethasone (D), and 2 μM insulin (I)), rosiglitazone, berberine, vitexilactone, vitexicarpin, and oleanolic acid are indicated by CTRL, MDI, ROS, BER, V, C, and O, respectively. Numbers with V, C, and O were concentration (μM) of each compound. On day 8 of culturing, the medium was removed, and cells were lysed using Ripa buffer. The triglycerol levels were determined by the Wako Triglycerol E-test (Wako Pure Chemicals, Osaka, Japan). Data are presented as the mean ± SD from three independent experiments. The same letters indicate that there are no differences between those groups, and different letters indicate significant differences (p < 0.05).