Figure 7.

The effects of each compound on adipogenesis-related proteins, adiponectin, and, GLUT4 levels in 3T3-L1 cells on day 8. The 3T3-L1 cells were cultured in 6-well plates and differentiated with DMI mixture with or without each compound under the conditions described in the Materials and Methods section and subsequently lysed with lysis buffer. Cell lysates were collected using a cell scraper and centrifuged at 15,000× g for 30 min at 4 °C. The supernatant was collected and the overall protein concentration was determined by a Protein Assay Reagent Kit (Cytoskeleton, Denver, CO, USA) with BSA as the standard. To detect GLUT4, membrane protein was extracted using Plasma Membrane Protein Extraction Kit according to the instructions of the manufacturer. Protein levels were measured by electroblotting. →, ↑ and ↓ represent no change, up- or down-regulation, respectively.