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. 2017 Oct 27;22(11):1840. doi: 10.3390/molecules22111840

Figure 4.

Figure 4

Effect of homopyrimidine bases of bisPNA and TC-PNA on complex formation with ssDNA. Each lane contains 100 nM of DNA1. (A) Each lane from the left side contains no PNA, 200 nM of PNA11, PNA12, PNA13, PNA14, PNA15, PNA16, and PNA17, which contain nine, eight, seven, six, five, four, and three homopyrimidine bases in the N-terminal strand of PNA, respectively. Each DNA and PNA were incubated in 10 mM sodium phosphate buffer (pH 6.0) at 25 °C for 10 min. Gel mobility shift assays of PNA/DNA complexes were performed using 15% non-denaturing polyacrylamide gels; (B) Quantitative band-intensity analysis of DNA and DNA/PNA complexes in Figure 4.