Figure 6.

Strand displacement of dsDNA by TC-PNA-C4-AZO. Each lane contains oligonucleotides as indicated above. The concentrations of DNA1 and DNA2 were 100 nM, while PNA15 was 200 nM. Each DNA and PNA were incubated in 10 mM sodium phosphate buffer (pH 6.0) at 25 °C for 10 min. Gel mobility shift assays of PNA/DNA complexes were performed using 15% non-denaturing polyacrylamide gels.