Table 1.
PNAs, TC-PNAs, and ssDNAs used in this study.
| Entry | Name | PNA Sequence (N to C)/DNA Sequence (5′ to 3′) | Mass | |
|---|---|---|---|---|
| Calcd. | Found | |||
| PNA1 | 12W | TCTCCCTTCTTT-Lys | 3266.44 | 3267.81 |
| PNA2 | 12W-C0-AZO | AZO-TCTCCCTTCTTT-Lys | 3549.80 | 3550.89 |
| PNA3 | 12W5H-(AEEA) | H-(Lys)3-CCTCT-(AEEA)-TCTCCCTTCTTT-Lys | 5082.51 | 5083.10 |
| PNA4 | 12W5H-(AEEA)2 | H-(Lys)3-CCTCT-(AEEA)2- TCTCCCTTCTTT-Lys | 5227.70 | 5228.86 |
| PNA5 | 12W5H-(AEEA)3 | H-(Lys)3-CCTCT-(AEEA)3-TCTCCCTTCTTT-Lys | 5372.80 | 5373.63 |
| PNA6 | 12W5H C0-AZO | H-(Lys)3-CCTCT-AZO-TCTCCCTTCTTT-Lys | 5220.74 | 5221.94 |
| PNA7 | 12W5H C3-AZO | H-(Lys)3-CCTCT-C3-AZO-TCTCCCTTCTTT-Lys | 5277.81 | 5278.77 |
| PNA8 | 12W5H C4-AZO | H-(Lys)3-CCTCT-C4-AZO-TCTCCCTTCTTT-Lys | 5291.82 | 5292.61 |
| PNA9 | 12W5H C5-AZO | H-(Lys)3-CCTCT-C5-AZO-TCTCCCTTCTTT-Lys | 5305.86 | 5306.59 |
| PNA10 | 12W5H C6-AZO | H-(Lys)3-CCTCT-C6-AZO-TCTCCCTTCTTT-Lys | 5319.89 | 5320.01 |
| PNA11 | 9W9H C4-AZO | H-(Lys)3-CTTCCCTCT-C4-AZO-TCTCCCTTC-Lys | 5528.17 | 5529.19 |
| PNA12 | 9W8H C4-AZO | H-(Lys)3-TTCCCTCT-C4-AZO-TCTCCCTTC-Lys | 5276.87 | 5277.21 |
| PNA13 | 9W7H C4-AZO | H-(Lys)3-TCCCTCT-C4-AZO-TCTCCCTTC-Lys | 5010.62 | 5011.17 |
| PNA14 | 9W6H C4-AZO | H-(Lys)3-CCCTCT-C4-AZO-TCTCCCTTC-Lys | 4744.37 | 4745.22 |
| PNA15 | 9W5H C4-AZO | H-(Lys)3-CCTCT-C4-AZO-TCTCCCTTC-Lys | 4493.07 | 4494.28 |
| PNA16 | 9W4H C4-AZO | H-(Lys)3-CTCT-C4-AZO-TCTCCCTTC-Lys | 4241.77 | 4242.89 |
| PNA17 | 9W3H C4-AZO | H-(Lys)3-TCT-C4-AZO-TCTCCCTTC-Lys | 3990.47 | 3991.77 |
| DNA1 | Cy3-CATCATCAAAGAAGGGAGATGGTG | 7982.50 | ||
| DNA2 | CCATCTCCCTTCTTTGATGATG | 6627.40 | ||
| DNA3 | Cy3-CATCATCAAATAAGGTAGATGGTG | 7932.40 | ||
AEEA: 2-aminoethoxy-2-ethoxy acetic acid, AZO: Thioazobenzene, Lys: Lysine. Underline: Mismatch bases.