Table 2.
PNA | Name | Tm | ΔTm * | ΔTm ** |
---|---|---|---|---|
1 | 12W | 68.0 | - | |
2 | 12W-C0-AZO | 72.9 | 4.9 | |
6 | 12W5H-C0-AZO | 81.5 | 13.5 | |
9 | 12W5H-C4-AZO | 79.3 | 11.3 | |
5 | 12W5H-(AEEA)3 | 86.3 | 18.3 | |
11 | 9W9H-C4-AZO | 79.6 | 19.9 | |
13 | 9W7H-C4-AZO | 74.3 | 14.6 | |
15 | 9W5H-C4-AZO | 68.0 | 8.3 | |
17 | 9W3H-C4-AZO | 59.7 | - |
* melting temperature difference relative to PNA1(12W)/DNA1; ** melting temperature difference relative to PNA17(9W3H-C4-AZO)/DNA1.