Skip to main content
. 2017 Oct 26;22(11):1822. doi: 10.3390/molecules22111822

Figure 4.

Figure 4

LSS-11 inhibits STAT3 phosphorylation and hinders STAT3 binding to MDR1 and MRP1 promoters. (a) Western blot analysis of p-STAT3 and STAT3 in A549 and A549/T cells treated with or without LSS-11 for 24 h. β-actin was used as loading control; (b) Quantification of immunoblot bands of p-STAT3 and STAT3 by ImageJ software. *** p < 0.001; (c) Pearson correlation efficient between suppression of p-STAT3 and downregulation of MDR1 or MRP1 by LSS-11; (d) Gene expression of MDR1 and MRP1 induced by STAT3 inhibitor sulforaphane. *** p < 0.001; (e) Apoptotic cells in A549/T cells treated with STAT3 inhibitor sulforaphane (20 µM) for 24 h as measured by annexin V/PI staining; (f) STAT3 ChIP-qPCR analysis displayed the binding sites of STAT3 in the promoter region of MDR1 (upper) and MRP1 (lower) genes. All experiments were performed in triplicates.