Schematic diagram of development of indel markers (AcoTT and AcoYN) in A. pseudolaeve Nakai, A. longecassidatum Nakai, and A. barbatum Patrin ex Pers. (A) Primers for AcoTT were tested in 13 Aconitum species. AcoTT length of A. barbatum was measured in the CP sequence; (B) Primers for AcoYN were validated in 13 Aconitum species: (1) A. barbatum, (2) A. pseudolaeve, (3) A. longecassidatum, (4) A. coreanum (H.Lév.) Rapaics, (5) A. carmichaelii Debeaux, (6) A. voluvile var. pubescens Regel, (7) A. jaluense var. triphyllum (Nakai) U.C.La, (8) A. kusnezoffii Rchb., (9) A. jaluense Kom., (10) A. austrokoreense Koidz., (11) A. monanthum Nakai, (12) A. kirinense Nakai, (13) A. chiisanense Nakai. * The CP genomes of A. pseudolaeve and A. longecassidatum were completed in this study. AcoYN: Aconitum ycf1-ndhF; AcoTT: Aconitum trnK-trnQ. CP: chloroplast.