Table 3.

Method validation for simultaneous quantification of 16 constituents in C. paliurus leaves.

Compound	Regressive Equation a	Linear Range (ug/mL)	R2	LOD (ng/mL)	LOQ (ng/mL)	Precision		Repeatability RSD% (n = 6)	Recovery
						Intraday RSD% (n = 6)	Interday RSD% (n = 18)		Mean	RSD% (n = 6 )
3-O-caffeoylquinic acid	y = 4943.9x − 37,710	8–640	0.9998	64.25	214.21	0.88	2.14	0.83	98.72	1.89
4-O-caffeoylquinic acid	y = 6245.2x − 14,821	4–192	0.9998	57.93	197.58	0.71	1.94	1.92	96.53	1.24
Quercetin-3-O-glucuronide	y = 23,136x − 119,324	2.5–600	0.9997	40.28	128.74	0.43	1.21	0.53	98.32	2.13
Quercetin-3-O-galactoside	y = 20,402x − 28,339	2–480	0.9994	52.94	174.17	0.48	1.59	0.92	102.43	0.92
Isoquercitrin	y = 21,231x + 2369.2	2–180	0.9997	58.42	192.52	0.98	2.35	1.89	97.89	2.02
Kaempferol-3-O-glucuronide	y = 16,702x − 92,304	2–640	0.9995	43.98	153.14	0.62	1.93	2.13	100.93	2.61
Kaempferol-3-O-glucoside	y = 18,066x − 87,126	2–480	0.9991	53.85	187.37	1.26	2.78	1.89	99.23	2.67
Quercetin-3-O-rhamnoside	y = 15,638x − 21,262	2–240	0.9993	62.48	199.32	0.49	0.98	0.57	98.92	1.95
4,5-di-O-Caffeoylquinic acid	y = 9428.7x +52,403	2.5–140	0.9997	58.97	201.22	0.63	1.52	0.82	101.29	1.82
Kaempferol-3-O-rhamnoside	y = 15,648x + 12,295	5–150	0.9997	64.13	211.81	0.58	1.68	1.52	97.69	1.91
Arjunolic acid	y = 5465.1x + 33,575	5–1500	0.9994	78.13	260.17	0.48	1.19	1.04	102.38	2.48
Cyclocaric acid B	y = 8579.6x − 91,948	7.5–1080	0.9993	90.16	296.36	0.92	2.83	2.05	102.9	2.52
Pterocaryoside B	y = 3716.1x + 52,647	6.7–1608	0.9991	72.46	210.27	1.23	2.42	2.04	98.19	2.61
Pterocaryoside A	y = 3746.1x + 56,466	10–1200	0.9991	86.39	268.62	0.84	1.96	1.29	102.85	1.85
Hederagenin	y = 6054.7x + 25,521	8–640	0.9997	72.82	232.3	0.91	2.53	1.83	101.83	1.69
Oleanolic acid	y = 7144.8x + 963.97	1–200	0.9994	58.41	185.72	1.23	2.59	2.43	98.28	2.93

^a y is the peak area, while x is the concentration of each analyte (ug/mL).