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. 2018 Sep 21;13(9):e0204408. doi: 10.1371/journal.pone.0204408

Fig 9. Detection of inoculated P solubilizing bacteria at 35 DAS.

Fig 9

Viable count after 35 days to study the survival of Pseudomonas sp. MS16 (A) and Enterobacter sp. MS32 (B) on the roots of wheat plants. Yellow, circular and shiny colonies indicated by red arrows show the presence of Pseudomonas sp. MS16 while Off-white, circular, and shiny colonies indicated by yellow arrows show presence of Enterobacter sp. MS32 among total indigenous bacterial soil population. Amplification of colony of isolated bacteria by using BOX primer in PCR (C). BOX-PCR of re-isolated colonies obtained from rhizosphere of plants grown in pots inoculated with; (A) Pseudomonas sp. MS16; Lane 1: 1 Kb DNA Ladder, Lane 2: Re-isolated colony of Pseudomonas sp. MS16; Lane: 3-6 Colonies of other isolates obtained, Lane:7 Negative control, Lane 8: 100bp DNA Ladder. (B) Enterobacter sp. MS32; Lane 1: 1 Kb DNA Ladder, Lane 2: Re-isolated colony of Enterobacter sp. MS32, Lane: 3-6 Colonies of other isolates obtained, Lane:7 Negative control, Lane 8: 100bp DNA Ladder.