Table 2. Morphology, identifition and characterization of P solubilizing bacteria for growth promoting traits.
| Strain | 1Identification | Accession no. | 2Colony Morphology |
2Cell Morphology |
3IAA Production (μg mL-1) |
4Gibberellic acid Production (μg mL-1) |
4ARA Activity (nmol ethylene h-1 mg protein-1) |
5Zinc Solubilization (SI) |
6ACC Deaminase Activity |
|---|---|---|---|---|---|---|---|---|---|
| MS16 | Pseudomonas koreensis | LT908012 | Yellow, circular, entire margins, shiny, convex | Long rods, motile | 25.6±1.40 | 2.5±0.70 | 8±0.2 | 2.8±0.18 | ++ |
| MS32 | Enterobacter cloacae | LT908013 | Off-white, circular, entire margins, shiny, convex | Small rods, motile | 28.1±1.23 | 11.8±3.11 | 19±0.3 | 3.3±0.12 | +++ |
1Bacteria were identified on the basis of 16S rRNA gene amplification and sequencing
2Colony and cell morphology of pure bacterial cultures were studied using light microscopy at 100X.
3Indole acetic acid (IAA) was quantified by HPLC.
4Acetylene Reduction Assay (ARA) activity quantified by GC.
5Zinc Solubilization detected by plate assay, Solubilization index (SI) on Pikoviskaya agar
61-Aminocyclopropane-1-carboxylic acid (ACC) deaminase activity was qualitatively studied by using ACC as a sole nitrogen source in DF minimal medium. + represents level of growth