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. 2018 Sep 21;13(9):e0204520. doi: 10.1371/journal.pone.0204520

Fig 4. A peptide with the ALG-2 binding sequence of ALIX impairs the ALG-2 pro-survival function.

Fig 4

A. HeLa cells expressing EGFP or EGFP-ALG-2 were treated with 50 μM digitonin in the presence of 10 μM ALIX peptide with ALG-2 binding sequence, ALIX mutant peptide incapable of ALG-2 binding, unrelated peptide or vehicle, as indicated. Cell viability was assessed by quantifying EGFP expressing cells (blue symbols) or cells expressing EGFP-ALG-2 (red symbols) one hour post-treatment. Individual data from at least three independent experiments are shown. Means +/- SEM are indicated. Statistical analysis was performed using unpaired t-test with Welch’s correction. B. Cellular uptake of TMRA-labeled ALIX peptides (top panels: wt ALG-2 binding ALIX peptide, bottom panels: mutated version incapable of ALG-2 binding) with the corresponding brightfield images in the absence (left panel) or the presence (right panel) of digitonin.