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. 2018 Sep 4;12(9):e0006769. doi: 10.1371/journal.pntd.0006769

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Fig 1 — (A) Needles were pulled using a Sutter P-2000 micropipette puller. (B) (C) Pulled needles were beveled to an angle of 20° using a Sutter BV-10 Microelectrode Beveller, 104D fine abrasive plate. (D) Needles were inspected under a microscope to ensure the bore is not greater than 1 μm. NOTE: If there is no availability of a needle beveller, needles can be opened by breaking the bore towards a glass slide under the microscope (always verify the opening is smaller than 1 μm).