Table 1. sgRNA targeting LuloYLW gene.

sgRNA	5’- T7-promoter Gene-specific (20mer) Scaffold-F (CRISPR) -3’	Genomic location	PAM	Strand	GC (%)	Self-complementarity	Off-targets	Efficiency
LuloYLW_1	GAAATTAATACGACTCACTATAGGATATGGGCAGGGATTCCTAGGTTTTAGAGCTAGAAATAGC	Scaffold614:35631	TGG	-	52	0	0	0.57
LuloYLW_2	GAAATTAATACGACTCACTATAGGGACATTGAGAGCATATGGGCGTTTTAGAGCTAGAAATAGC	Scaffold614:35643	AGG	-	52	0	0	0.62
LuloYLW_3	GAAATTAATACGACTCACTATAGGTCATATTGGTGTCCTCAGGTGTTTTAGAGCTAGAAATAGC	Scaffold614:35707	CGG	-	52	0	0	0.49
LuloYLW_4	GAAATTAATACGACTCACTATAGGGCGCTACGTTCTCCGACCTGGTTTTAGAGCTAGAAATAGC	Scaffold614:35695	AGG	+	65	0	0	0.57
LuloYLW_5	GAAATTAATACGACTCACTATAGGCTGATGTCTGCGGTAGGCTTGTTTTAGAGCTAGAAATAGC	Scaffold614:35574	TGG	+	57	0	0	0.49
LuloYLW_6	GAAATTAATACGACTCACTATAGGCGCATCAAGGCTGATGTCTGGTTTTAGAGCTAGAAATAGC	Scaffold614:35564	CGG	+	61	1	0	0.46
Scaffold-R	AAAAGCACCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATTTTAACTTGCTATTTCTAGCTCTAAAAC