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. 2018 Apr 13;10(5):712–719. doi: 10.1080/19420862.2018.1463122

Figure 2.

Figure 2.

Fluorescence imaging of Fab and IgG antibodies modified by 6-AM-2-PCA. (A) Fab antibodies were treated with 6-AM-2-PCA, following by fixing to the membrane and incubation with DBCO-Seta650. Fluorescence signal was detected. (B) IgG antibodies were treated with 6-AM-2-PCA, following by fixing to the membrane and incubation with DBCO-Cy5.5. Fluorescence signal was detected. (C) IgG antibodies were treated with 6-AM-2-PCA, and sequentially labeled with DBCO-Cy5.5. The upper panel is the fluorescent imaging. The lower panel is Coomassie brilliant blue R-250 staining of proteins.