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Characterization of anti-CD33 mAb binding to stable 293 HEK cells overexpressing recombinant human (red) and cynomolgus monkey (black) CD33. Incubations performed on ice for 30 min followed by detection with a goat-anti-human IgG-PE secondary reagent. Approximate EC50 for human and monkey are 0.60 µg/ml and 0.35 µg/ml, respectively.
