Table 2.

Retention of anti-Aβ-LS-anti-TfR1(AB221) DVD-Ig in brain for 96 hours after subcutaneous dosing. Mice injected subcutaneously with anti-Aβ-LS-anti-TfR1(AB221) DVD-Ig at 20 mg/kg or 50 mg/kg either once (at 0 hour) or at 40 mg/kg twice (0 and 48 hours) before processing at 96 hours. Serum anti-Aβ-LS-anti-TfR1(AB221) DVD-Ig concentrations were measured at indicated times post injection, except for 40 mg/kg dose second injection at 48 h was performed right after serum collection at 48 h. Note an anti-Aβ-LS-anti-TfR1(AB221) DVD-Ig brain concentration of 1.6 ± 0.20 nM was retained at 96 hours after a single injection. Data was expressed as means ± SD, n = 4 mice per group, except where indicated with *n = 3.

Treatment	Collection Time. (hr)	Serum conc. (nM)	Brain conc. (nM)
20 mpk	1	3.7 +/− 3.1
	24	142.5 +/− 3.2
	48	33.7 +/− 12.4
	72	8.0 +/− 2.8
	96	1.7 +/− 0.2	1.6 +/− 0.2
50mpk	1	11.2 +/− 7.9
	24	679.8 +/− 142.0
	48	187.3 +/− 67.2
	72	94.6 +/− 36.4
	96	6.1 +/− 1.7	6.9 +/− 2.6
40 mpk (at 0 and48 hr)	1	3.2 +/− 2.5
	24	109.9 +/− 16.7*
	48	20.0 +/− 1.6
	72	365.0 +/− 189.1
	96	95.6 +/− 70.2	9.0 +/− 2.9