Figure 2.

Optimization of exercise regimen for parkin- males using the climbing assay. Parkin- males had highest climbing index when exercised for 30 mins every alternate day (Regimen 4). Regimens 1, 2 and 3 exercised parkin- for 30, 60 and 120 mins every day, respectively. Regimens 4 and 5 exercised parkin- males for 30 and 60 mins, respectively on alternate days. Each regimen had 6 replicates of 12 parkin- males for 72 parkin- males/regimen (a total of 432 flies were included in the study). The flies were fed 1:2 P:C ratio diet throughout the exercise period. Bars indicate means and error bars indicate standard error. Significance determined by Dunnett’s test at *P < 0.05, **P < 0.01 (see text for details).