Figure 3.

Probe 4 irreversibly binds to hA_2AR HEK293 cell membranes stably expressing hA_2AR, and they were preincubated with probe 4 (a) or ZM241385 (b) at the indicated comparable concentrations. Pretreated membranes were washed three times extensively before further displacement studies of specific [³H]ZM241385 binding from the hA_2AR at 25 °C by nonlabeled ZM241385 were performed. Representative graphs are from three independent experiments performed in duplicate with error bars representing SEM values.