Figure 1.

Trapping proteins inside the ClyA nanopore. (Left) Surface representation of Type I ClyA-AS (blue) in the bilayer (gray) with SBD1 (PDB-ID = 4KPT) in open conformation lodged inside the nanopore. (Right) SBD2 in open (PDB-ID = 4KR5) and closed (PDB-ID = 4KQP) conformation. SBDs are colored according to the residue type: basic residues are colored blue, acidic residues red, polar residues green, and nonpolar residues white. Created with VMD²⁴ while ClyA was created by homology modeling using the E. coli ClyA crystal structure.^16,25