Figure 4.

Surface-immobilized encapsulin as a bionanoreactor. (A) Immobilization strategy of encapsulin particles on a glass surface using PFPS molecules as linkers. (B) SEM image of surface-immobilized encapsulin showing the presence of nanometer-sized particles (∼24 nm) on the surface. (C) Schematic representation of a tandem system consisting of GOx and DyP-E. The initial substrate glucose is oxidized by GOx while producing H₂O₂, which in turn is converted by DyP into water while producing green-colored ABTS radical. (D) Catalytic assay of the tandem system monitored at λ = 410 nm based on the absorption of the ABTS radical cation.