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. Author manuscript; available in PMC: 2019 Sep 1.
Published in final edited form as: J Mol Neurosci. 2018 Aug 14;66(1):121–134. doi: 10.1007/s12031-018-1154-7

Fig. 5.

Fig. 5

The effects of a CB2 antagonist (AM630) on neuroprotection provided by either cannabidiol or KLS-13019 were compared in hippocampal cultures co-treated with 30 mM ethanol. The responses were assessed with the CFDA neuronal viability assay. Each value is the mean of 5 determinations ± the standard error of a representative experiment. Various concentrations of AM630 were added to cultures co-treated with either 10 μΜ CB (closed circles) or 100 nM KLS-13019 (open circles). In cultures treated with 10 μΜ CBD, the concentration-effect study of AM630 revealed significant (P < 0.02) decreases in neuroprotection at concentrations ranging from 1 to 100 nM. Reference lines are used to show the mean values for control cultures (dot-dashed) and those cultures treated only with 30 mM ethanol (dashed line). At all of the concentrations tested for AM630, no significant differences from controls values were observed in cultures treated with 100 nM KLS-13019 plus 30 mM ethanol.