Table 1.

Real-time RT-PCR oligonucleotide primer sequences and cycling conditions

Accession no.	Gene	Sense (3′-5′)	Antisense (5′-3′)	Amplicon length (bp)	Annealing temperature (°C)	Annealing time (s)
NM_001101	β-actin	ATGACTTAGTTGCGTTACAC	AATAAAGCCATGCCAATCTC	79	58	10
NM_000479	AMH	GTGCTGCTGCTGAAGATG	CTCCGACAGGCTGATGAG	102	62	10
NM_001164690	AMH-R2	CCAGAAGCACGGCTGACAG	TGGAAAGGGGTGGCTCTCT	81	62	10
NM_001288824	GDF9	TCGCATTACTACCGTTGAA	CACACATTTGACAGCAGAG	91	53	10
NM_024865	NANOG	ACTCTCCAACATCCTGAA	TTCTGCCACCTCTTAGAT	84	55	30
NM_203289	OCT4b	AGGCACTTCTACAGACTATT	CACACCAGTTATCAATCTCC	82	58	30
NM_003106	SOX2	GGATGGTTGTCTATTAACTT	TCAAACTTCTCTCCCTTT	153	53	30
NM_003376	VEGF	GGAAACCAGCAGAAAGAGG	AAGCAGGTCACTCACTTTG	132	59	10