Table 2.
The Hippo gene expression profiles of cumulus cells without culture (static) compared to those cultured on platforms that produce variable mechanical forces. Only those values with three arrows show a significant change. Changes depicted with one or two arrows depict a general change in direction of expression. The mean difference compared to an ActinB housekeeping gene (ACTB) is provided along with the p value in parentheses. Control static cells from eight patients were compared to those plated on standard polystyrene tissue culture dishes (donor plastic), an inert 3D biologic scaffold (donor scaffold), and the biological scaffold on a dynamic piston device which created a convex displacement of the scaffold (donor scaffold stretch)
| Donor genes static | Donor plastic | Donor scaffold | Donor scaffold stretch | |||
|---|---|---|---|---|---|---|
| LATS1 | 0.833 (0.25) | ↑ | 0.334 (0.64) | – | 0.624 (0.38) | ↑ |
| LATS2 | 0.916 (0.26) | ↑ | 0.793 (0.33) | ↑ | 0.626 (0.44) | ↑ |
| YAP1 | 1.584 (0.05) | ↑↑↑ | 0.416 (0.60) | – | 1.543 (0.05) | ↑↑↑ |
| WWTR1 | 1.459 (0.06) | ↑↑ | 0.084 (0.91) | – | 0.291 (0.71) | – |
| MOB1A | − 0.959 (0.29) | ↓ | 0.375 (0.68) | – | − 0.918 (0.31) | ↓ |
| MOB1B | 0.291 (0.65) | – | − 0.207 (0.75) | – | 0.168 (0.80) | – |
|
ACTB
Control |
− 0.209 (0.80) | 0.166 (0.84) | 0.041 (0.96) | |||
↑↑ Non-significant (0.05 < P < 0.1) and ↑ (0.1 < P < 0.5) upregulation compared to static non-cultured donor controls
↓ Non-significant (01 < P < 0.5) downregulation compared to static non-cultured donor controls
↑↑↑ Statistically significant (p < 0.05) upregulation compared to static non-cultured donor controls