Table 2.
Differentially expressed genes when comparing the transcriptomes of granulosa cells from primordial follicles with that of granulosa cells from primary follicles
| DEG annotated ‘mitochondrial dysfunction’ pathway | |||||||
|---|---|---|---|---|---|---|---|
| Gene name | Gene symbol | GCs from PDF mean FP KM value | p value | GCs from PMF mean FPKM value | p value | Significance (paired t test) | Fold change down |
| Significantly downregulated in GCs during primordial-to-primary transition | |||||||
| Caspase 9 | CASP9 | 0.35730C941 | 0.191902227 | ND | ND | . | – |
| Leucine-rich repeat kinase 2 | LRRK2 | 1.742929277 | 0.18882465 | 0.152619841 | 0.422649731 | 0.202859976 | 11.42007003 |
| Xanthine dehydrogenase | XDH | 2.709244357 | 0.132521709 | 0.694350157 | 0.422649731 | 0.280037462 | 3.901841698 |
| Caspase 8 | CASP8 | 3.658460561 | 0.085398141 | 1.013842009 | 0.330037104 | 0.150990301 | 3.608511511 |
| NADH/ubiquinone oxidoreductase subunit B10 | NDUFB10 | 3.008997935 | 0.060705184 | 0.877799646 | 0.25403594 | 0.052881546 | 3.427886932 |
| SURF1, cytochrome C oxidase assembly factor | SU RF1 | 2.314066164 | 0.183536671 | 0.751575033 | 0.388497206 | 0.270072995 | 3.078955608 |
| Apoptosis inducing factor, mitochondria associated 1 | AIFM1 | 3.613715001 | 0.044079763 | 1.417073168 | 0.213958626 | 0.240541281 | 2.550125909 |
| Presenilin 2 | PSEN2 | 3.263341286 | 0.024146621 | 1.400426297 | 0.40453294-9 | 0.203847324 | 2.330248505 |
| Mitogen-activated protein kinase 9 | MAPK9 | 3.075312273 | 0.1845136 | 1.441810509 | 0.422649731 | 0.358332488 | 2.135726056 |
| ATP synthase, H+ transporting, mitochondrial F1 complex, gamma polypeptide 1 | ATP5C1 | 3.989803o98 | 0.039197685 | 1.904638856 | 0.312564394 | 0.291986732 | 2.094782266 |
| Thioredoxin 2 | TXN2 | 2.058542691 | 0.093586399 | 1.00385669 | 0.309227225 | 0.409842284 | 2.0S0634032 |
| Significantly upregulated in GCs during primordial-to-primary transition | |||||||
| NADH/ubiquinone oxidoreductase core subunit S8 | NDUFS8 | ND | ND | 3.123248644 | 0.051406125 | – | – |
| Ras homolog family member T2 | RHOT2 | 0.347494344 | 0.422649731 | 1.838057874 | 0.199400508 | 0.263934146 | 5.289461278 |
| Cytochrome B5 type A | CYB5A | 1.166231252 | 0.163820909 | 3.106697839 | 0.141002458 | 0.206470227 | 2.663878055 |
Eleven transcripts were significantly (fold change > 2) downregulated, whilst three were significantly upregulated (fold change > 2) in the granulosa cells during the primordial-to-primary follicle transition
ND not determined