Fig. 1.

Examination of the consistency of granulosa cell (GC) number per follicle in a donor. a 12 randomly selected AFs were aspirated (solid arrow) from each gilt ovary to obtain GC. The aspiration was repeated to obtain two sets of GCs from each gilt. Seventeen gilts were used for this comparison. The GC number per follicle was determined using real-time PCR. b There was a significant correlation between the GC number of the two sets (R = 0.59, P < 0.05). Based on this result, we measured the GC number for each gilt using 20 AFs (dotted arrow in a). c Average granulosa cell number in the follicles of donors. Granulosa cells were collected from 20 AFs from each gilt. Two hundred sixty-five gilts were examined. X axis represents GC number and Y axis represents the number of donors