Fig. 3.

Relationship between the number of granulosa cells (GCs) in follicles and the developmental rate (at day 7) to the blastocyst stage (%) of oocytes among donors. Oocytes were collected from all middle size follicles (3–5 mm in diameter) from each donor, and all oocytes (25–40 for each donor) were used to determine the developmental rate to the blastocyst stages. The GC number per follicle was determined using the following formula: GC number per follicle = (copy number of single copy gene / 2) / number of follicles aspirated. GC number and developmental rate to the blastocyst stage were compared among 41 donors