FIG. 4.

In vitro SC encapsulation in a 20/80 GelMA/MeTro hydrogel at 10% (w/v) total polymer concentration. (A) Representative calcein-AM (green)/ethidium homodimer (red) stained images from cell-laden hydrogels after 1 and 5 days of culture. (B) Quantification of cell viability after 1, 3, and 5 days postencapsulation. (C) Representative phalloidin (green)/DAPI (blue) stained images from cell-laden hydrogels after 1 and 5 days of encapsulation. (D) Quantification of total cell number by nuclei counting after 1, 3, and 5 days of encapsulation. (E) Representative immunofluorescent image showing the expression of SC marker S100 (red)/DAPI (blue) 5 days after encapsulation. (F) Quantification of metabolic activity (RFU) by PrestoBlue 1, 3, and 5 days postencapsulation (*p < 0.05, **p < 0.01, ****p < 0.0001). DAPI, 4′,6-diamidino-2-phenylindole; RFU, relative fluorescence units. Color images available online at www.liebertpub.com/tea