FIG. 5.

In vitro dorsal root ganglia encapsulation within GelMA/MeTro hydrogels. (A) Representative β-3-tubulin (purple) immunofluorescent images of encapsulated dorsal root ganglia in GelMA, MeTro, 50/50 GelMA/MeTro at 10% (w/v) total polymer concentration and 30 s UV exposure. (B) Quantification of total outgrowth area from MATLAB image analysis (n > 4). (C) Representative high magnification immunofluorescent images of axonal extension in 50/50 GelMA/MeTro at 10% (w/v) total polymer concentration, β-3-tubulin, and SC morphology, S100 (red), and cell nuclei (cyan). (ns: p > 0.05, ****p < 0.0001). Color images available online at www.liebertpub.com/tea