Fig. 3.

Proliferation of pMSCs and HUVECs in response to various concentrations of glucose. MTS proliferation assay showed proliferation of pMSCs in response to 20, 40, 60, 80, 100, and 150 mM glucose did not significantly change as compared to glucose-untreated pMSCs after 24 h (a), 48h (b), and 72h (c) while treatment with 200–2000 mM glucose for 24 h (a), 48 h (b), and 72 h (c) significantly decreased proliferation of pMSCs as compared to glucose-untreated pMSCs. HUVEC proliferation in response to 20 and 50 mM glucose did not significantly change as compared to glucose-untreated HUVECs after 24 h (d), 48 h (e), and 72 h (f). Culture of HUVECs with 100 mM glucose for 24 h (d) did not significantly change proliferation, but significantly decreased proliferation after 48h (e) and 72 h (f) as compared to glucose-untreated pMSCs. HUVEC proliferation in response to 200 mM glucose significantly reduced as compared to glucose-untreated HUVECs after 24 h (d), 48 h (e), and 72 h (f). Each experiment performed in triplicate using pMSCs (passage 2) and HUVECs (passage 3–5) from five independent placentae and umbilical cord tissues. *p < 0.05. Bars represent standard errors