Fig. 5.

HUVEC adhesion in response to glucose and pMSCs, or after removing effects of glucose and pMSCs. HUVECs cultured with 100 mM glucose alone (100), or with 25% CMpMSC (100 + CM), and adhesion then measured using xCELLigence real-time cell analyser. After 2 h, as compared to glucose-untreated HUVECs, HUVEC adhesion in presence of glucose alone (100), or with CMpMSC (100 + CM), did not significantly change (p > 0.05) (a). Adhesion of HUVECs in presence of glucose and CMpMSC (100 + CM) did not significantly change as compared to glucose-treated HUVECs (100) after 2 h (p > 0.05) (a). HUVECs pretreated with 100 mM glucose (100(pre)) in presence of different pMSC treatments (CMpMSC (PreCM + 100) and ICpMSC (PreIC + 100)) were cultured in 16-well culture plate and adhesion measured as already indicated. After 2 h, and as compared to glucose-untreated HUVECs, preteatment of HUVECs with glucose (100(pre)), or with glucose and CMpMSC (PreCM + 100), or with glucose and ICpMSC (PreIC + 100), did not significantly change (p > 0.05) (b). Adhesion of HUVECs in presence of glucose and CMpMSC (PreCM + 100), or glucose and ICpMSC (PreIC + 100), did not significantly change as compared to glucose-treated HUVECs (100(pre)) after 2 h (p > 0.05) (b). Each experiment performed in triplicate using HUVECs (passage 3–5) and pMSCs (passage 2) from five independent umbilical cord tissues and placentae, respectively. Bars represent standard errors